Targeted DISCOvery of novel cellulases and hemicellulases and their reaction mechanisms for hydrolysis of lignocellulosic biomass

akronüüm: DISCO
algus: 2008-08-01
lõpp: 2012-07-31
 
programm: FP7 - Euroopa Liidu 7. raamprogramm
alaprogramm: KBBE - Toit, põllumajandus ja biotehnoloogia
instrument: CP-FP - Väikese- ja keskmisemahulised koostööprojektid
projektikonkurss: FP7-KBBE-2007-1
projekti number: 211863
kestus kuudes: 48
partnerite arv: 11
 
lühikokkuvõte: The aim of the proposed DISCO project is to develop more efficient and therefore more cost-effective cellulosic and hemicellulosic enzyme tools for the enhanced hydrolysis of pre-treated lignocellulosic biomass in simultaneous saccharification and fermentation (SSF) conditions for bioethanol production. The focus will be on enzymes having increased catalytic activity on various types of relevant European lignocellulosic biomass. In addition enzymes with lower affinity for lignin shall also be developed. Such enzymes would increase the effective amount of cellulases/ hemicellulases for cellulose hydrolysis. Furthermore the recycling of these enzymes would be applicable. The approach in this proposal is to discover the desired activities by combining classical and modern screening technologies. The enzymes will be produced in suitable host systems for industrial enzyme production. The project also focuses on elucidation of enzymatic hydrolysis mechanisms, about which there is a paucity of knowledge. The project will determine the limiting structural factors in these mechanisms by characterisation of the substrate during the course of the hydrolysis and the remaining recalcitrant residue. Synergy between different cellulase and hemicellulases components will also be addresses on the chosen lignocellulosic substrates. Furthermore, the project seeks to demonstrate the proof of concept with the cellulolytic enzymes in a pilot scale using the most relevant European feedstock pretreated wheat straw and related high-volume co-products.
partneri jrk nr ja roll partneri nimi riik kontaktisik koduleht
1 koordinaator VALTION TEKNILLINEN TUTKIMUSKESKUS FI Riitta Kervinen http://www.vtt.fi
2 partner WAGENINGEN UNIVERSITEIT NL Jos Teunissen http://www.wageningenuniversiteit.nl/UK/
3 partner INSTITUTE OF FOOD RESEARCH UK Mary Anderson http://www.ifr.ac.uk
4 partner HELSINGIN YLIOPISTO FI Sanna-Maija Miettinen http://www.helsinki.fi/university
5 partner Institution of the Russian Academy of Sciences, A.N. Bach Institute of Biochemistry of RAS RU Vladimir Popov http://www.inbi.ras.ru
6 partner BUDAPESTI MUSZAKI ES GAZDASAGTUDOMANYI EGYETEM HU Miklós Zrínyi http://www.bme.hu
7 partner DYADIC NEDERLAND BV NL Wim Van Der Wilden
8 partner Biogold OÜ EE Priit Kotli http://www.biogold.ee
9 partner G R WRIGHT AND SONS LTD UK Lesley Morris
10 partner SEKAB E-TECHNOLOGY AB SE Torbjörn Van Der Meulen http://www.sekab.com
11 partner AGRIFIRM GROUP BV NL Albert Jan Van Dijk http://www.agrifirm.com